Tissue culture Sheep Pox Vaccine(Living)

Definition:-

• It is a live attenuated freeze-dried vaccine prepared by growing attenuated Sheep pox virus in lamb kidney/thyroid cell culture.

Use:-


• It is used in sheep only, for protection against Sheep Pox. It can be used both for clean vaccination and in face of an outbreak.
• Primary Vaccination:- 5 to 7 days of age.
• Booster vaccination:- 5th week of age.

Doses and Administration:-


• 0.5 ml. of reconstituted vaccine by subcutaneous route on the external aspect about an inch from the tip of the ear. Care should be taken to avoid vaccine going into blood vessels.

Reactions:-


• A nodular thickening develops at the site of vaccination within a week and persists for a long time. In, few cases nodules may break leading into ulceration which heal with dressing by use of boro-glycerine.

Immunity:-


• It lasts for one year.

Precautions:-

• Transport of vaccine on ice is essential to maintain cold chain.
• Store in freezing chamber of the refrigerator.
• Reconstitute carefully in a chilled diluent, and mix well before administration.
• Use within 2 hours after reconstitution. Keep on ice.
• Do not apply Tr. iodine or spirit while vaccination.
• Use sterile syringes and needles.
• Shake the bottle every time when the vaccine is drawn.

Storage:-


• • At -150 C to -200 C..............One year from the date of manufacture.
  • At 00 C to 40 C ......................One month.

Packing:-


• Ampoule / vial containing 25/50/100 doses. Diluent - Aluminium hydroxide gel 100 ml.vial.

Reconstitution:-


• The contents of each ampoule/vial are reconstituted in chilled diluent supplied by this institute. The 100 dose ampoule is mixed in 50 ml. of diluent and shaken vigorously for 1 to 2 minutes and allowed to stand for 15 to 20 minutes in refrigerator or on ice. This will allow the virus to adsorb in a diluent. This mixture is to be used within two hours of reconstitution.